Dissecting protein dynamics in living cells by FRAP Back

Date 5th November 2015Time 16:00Presenters Dr. Marco Frietzsche and Jennifer Horner

In this webinar you will learn about the use of Fluorescence Recovery After Photo-bleaching (FRAP) microscopy to study protein dynamics. It will be presented by Dr Marco Fritzsche, University of Oxford, and Jennifer Horner, PhD, Leica Microsystems.

Marco will take a look at the advantages of FRAP microscopy to study the binding dynamics of proteins associated to the cytoskeleton and the plasma membrane of living cells. He will discuss sample preparation, calibration, and application of an optimal FRAP protocol as well as give an overview of the type of protein dynamics that can be measured by FRAP.

The broadly applicable method based on FRAP he will present answers the following questions:

  • How many reaction processes participate in the turnover of any given protein of interest?
  • How are their apparent association and dissociation rates characterized?
  • What is their relative importance in the turnover of the overall protein population?
  • How to identify the protein domains that mediate each of the identified turnover processes?

References:
Fritzsche et al. Nature Protocols 2015
Fritzsche et al. BiophyJ 2014
Fritzsche et al. MBoC 2013

Jennifer will give a brief introduction to the Leica DMi8. This open and freely configurable inverted research microscope for live cell imaging is equipped with an additional incident illumination port. This so-called Infinity Port facilitates the integration of additional light sources and laser systems for advanced applications, including FRAP.

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