From Epifluorescence to Superresolution in 3D: Exploring the secrets of cellular logistics Back

Date 4th June 2013Time 16:00Presenters Prof. Dr. Ralf Jacob and Dr. Nathalie Garin

The plasma membrane of epithelial cells is divided into separate membrane compartments, the apical and the basolateral domain. This polarity is maintained by intracellular machinery that directs newly synthesized material into the correct target membrane. Moreover, glycoproteins and lipids can enter distinct pathways for apical or basolateral delivery. To discover these pathways and unravel the complex network of post Golgi endosomal apical trafficking a combination of biochemical and microscopic approaches was used.

This webinar will illustrate results obtained by biochemical, Epifluorescence, TIRFM, Confocal and GSD techniques. Depending on the aim of experimental question, different imaging techniques deliver insights into varying aspects of intracellular pathways.

To achieve “True-to-detail imaging” of the spatial arrangement of proteins and other biomolecules in cells, GSDIM achieves resolutions up to 20nm in x and y direction - beyond the diffraction limit of light microscopy. But Super-resolution microscopy can be applied in the axial (z-) direction, too. A recent commercial implementation of the astigmatism approach will be discussed in more detail during this webinar…