Fluorescence Lifetime Imaging Made Easy... Back

Date 28th June 2016Time 16:00Presenters Dr. Gerhard Holst

Lifetime or decay rate relates to the phenomenon of fluorescence, the spontaneous emission of light that appears after excitation of a sample. This process, photoluminescence, is widely utilized across many areas, for tagging cells and cell fragments in order to observe metabolic processes, for differentiating between reaction products or for the characterization of any parameters that induce a change in the fluorescence, for example oxygen partial pressure.

Although the benefit of using the fluorescence lifetime as additional analytical parameter is known for years, except single point measuring devices, which are connected to scanning applications, and some image intensifier based systems, it has not been used on a broader scale. If the reason for this has been the complexity of the systems, we would like to suggest with this webinar a new approach which is less complex to handle.

In the webinar the Fluorescence Lifetime Imaging in the frequency domain with a dedicated camera system is described, from the background theory to the practical use. First the differences between time domain and frequency domain fluorescence lifetime measurements are explained and the special CMOS image sensor is introduced. The camera systems and its main features and limitations are described, followed by the first experimental data (FRET and endogenous fluorescence), which have been obtained. Finally some considerations about the application are presented and the performance is compared to alternative methods and discussed.

In case you would like to measure the luminescence lifetimes for FRET, for calibration of optical chemical sensors, for endogenous fluorescence differentiation, or if you are looking for dynamic changes of this parameter the introduction into the new measuring system could be relevant to your application.

Who should attend?
Everybody interested in measuring fluorescence lifetimes with a camera system.