Screening blood samples from race horses for prohibited drugs requires validated methods and instrumentation that maximize sensitivity and selectivity while minimizing sample handling. For this purpose, several methods were developed and validated based on liquid chromatography with mass spectrometry (LC-MS) utilizing a two-dimensional LC system that automates sample cleanup by TurboFlow™ technology.
We recently evaluated the latest version of this type of system, the Transcend™ II, which permits coupling TurboFlow chromatography to ultra high-resolution chromatography (UHPLC) at operating pressures as high as 1000 bar. In this webinar, experiences in transferring and validating methods from a legacy Aria system with TurboFlow methods to the Transcend II system, as well as developing and validating new drug screening methods, will be presented.
Key learning objectives
- Automating sample preparation steps for drug screens
- Avoiding potential sample adulteration during drug screens
- Improving sample throughput and data quality in drug screens
- Economizing drug screens
Who should attend
- Bio-analytical laboratory managers, supervisors & chemists
- Toxicology laboratory managers, supervisors & chemists
- Equine testing laboratory managers, supervisors & chemists
Joe Di Bussolo Joe Di Bussolo earned his B.S. degree in clinical chemistry at West Chester University in 1977. In 1982, he received a Ph.D. degree in biological sciences from Drexel University in Philadelphia. During those college years, Joe worked part time as a medical laboratory technologist at Children’s Hospital of Philadelphia. For most of the 20 years that followed, Dr. Di Bussolo helped develop and supported various analytical instruments and software products for the PerkinElmer Corporation. In 2002, Joe joined Cohesive Technologies as an applications scientist. Cohesive was acquired in 2007 by Thermo Fisher Scientific and Joe currently works under the Thermo umbrella as a senior scientist training and collaborating with customers and managing the Applications Laboratory at West Chester University of Pennsylvania. He is also an adjunct professor and advisor in the University's Pharmaceutical Product Development program.
Carisa Dixon Tate Carisa Dixon Tate graduated from West Chester University in 2004 with B.S. degrees in Forensic Chemistry and General Biology. In her senior year, Carisa completed an internship at the Pennsylvania Equine Toxicology and Research Laboratory (PETRL). Since 2008, she has been employed by the University of Pennsylvania’s School of Veterinary Medicine, New Bolton Center, as a research scientist for clinical studies assigned to PETRL where she analyzes race-horse samples as well as develops and validates new screening and conformation methods to detect and measure drugs by LC-MS.