Using Active Illumination microscopy techniques for deeper investigations into cell and systems biology Back

Date 3rd April 2014Time 16:00Presenters Dr Geraint Wilde

This webinar will introduce a range of active illumination techniques, highlighting published research that demonstrates the deeper insight these methods can bring to cell biology. Examples covered will include methods used for cell signalling, intracellular dynamics, damage and repair, neurophysiology and optophysiology (optogenetics).

The use of a microscope as part of a research project is now common place. Furthermore, it has progressed significantly from the simple visualisation of a fluorescently labelled target protein in a fixed sample, to the routine use of fluorescent proteins, such as green fluorescent protein (GFP), in live cell imaging. For example, through labelling multiple proteins of interest from the same signalling pathway, it is possible to follow, in real-time, their spatial distribution and temporal presence in relation to each other; watching how one signal transduction protein may be degraded, so releasing its bound partner to translocate to another cellular compartment. A great deal can be learnt from this passive observation on a microscope, extending what we discover from bench-based experiments. However, there is so much more to be investigated and revealed with a microscope, using techniques that either actively perturbs cell physiology, or that target specially designed light sensitive probes.

Learning Objectives:

  • Identify a range of techniques that can be added to a microscope for deeper investigation into cell and systems biology
  • Highlight key applications that benefit from Active Illumination techniques
  • Gain insight into the technology available

Who should attend?

  • Researchers wishing to manipulate cellular physiology in real-time
  • Core facility managers or those using multi-modal imaging
  • Those with an interest in live cell imaging