Imaging is one of the key technologies for Biomedical research. In many cases it is useful to obtain data from different imaging modalities. Especially combining 2 techniques in one single experiment, so-called Correlative Microscopy, can and should give a better answer than each technique alone. 1 + 1 = 3. Correlative Light Electron Microscopy (CLEM) can be defined as the “Observation and analysis of one and the same event by both a light and electron microscopy technique”.
There are many approaches to CLEM depending on the question to be answered. In some cases it may be to find specific cells, e.g. transfected or diseased in a large population. Our main interest is in intracellular trafficking. In our approach an event is therefore first imaged live in the light microscope to acquire the history of an event. The sample is then fixed and analysed in the electron microscope. The same event can now be analysed at higher resolution and in addition EM provides us with the surrounding ultrastructure as a reference space.
A CLEM experiment can in general be divided in 3 steps: probes, processing, and analysis. In the webinar I will highlight aspects of each these steps and focus on the Leica EMPACT2 + RTS and its use in the CLEM workflow.